For high-throughput mRNA profiling, we used the novel LightCycler® 1536 Real-Time PCR System. To evaluate the performance, accuracy, and speed of this new system, all genes measured in the previous analysis (using a competitor system) were also included for comparison.
The LightCycler® 1536 System is designed for high-throughput gene detection, gene expression, and genetic variation analysis. The LightCycler® 1536 Instrument facilitates mono- and dual-color applications using Universal ProbeLibrary (UPL) probes, hydrolysis probes, and intercalating dyes (e.g., BrightGreen). The instrument’s software provides basic PCR results (e.g., Cp’s, endpoint fluorescence values).
While the reproducibility of the results was found to be highly comparable on both systems, the novel LightCycler® 1536 Instrument allowed for higher sample throughput in a short time and reduced volumes of reagents and samples.